Anthelmintic Effects of Different Extracts of Hopea odorata Leaves on Tubifex tubifex Worm Using In vitro Method and their Condensed Tannin Content

Objective: To investigate the therapeutic effects of different extracts of Hopea odorata leaves in anthelmintic (In vitro) and to determine their total condensed tannin (proanthocyanidin) content.

Methods: Leaves of Hopea odorata was extracted with pure methanol (MEHO), ethanol (EEHO) and water (AEHO), which are tested for anthelmintic activity on aquarium worm Tubifex tubifex by using four concentrations viz., 2.5, 5, 10 and 20 mg/ml of each. Total condensed tannin content determined based on the previous procedure of Oyedemi et al. [1].

Result: Among the all extracts, MEHO exhibited strong anthelmintic activity In vitro. Where it paralyzed (4.05±0.35 min; P<0.05) and produced death (7.5±0.38 min; P<0.001) of the Tubifex tubifex at highest 20 mg/ml dose close to the value of the standard, Levamisole (3.3±0.38 min and 6.5±0.76 min) at 1 mg/ml. The content of condensed tannin excellent at all extracts, but MEHO (96.99±0.34 mg catechin/g) contained maximum among them. For both of experiment, activity found as follows, MEHO > EEHO > AEHO.

Conclusion: These findings suggest that the plant may be a potential source for the development of new anthelmintic and condensed tannin is a type of phytochemical which may exhibit anthelmintic activity.